Module number 2796




Database revision : gnsdb28.10
Date : Tue Feb 25 17:37:16 2003
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mYLR346C:Unknown ,, Unknown\n mPBP1:Poly(A)-binding protein binding protein,,Null mutant is viab\nle; other mutant suppresses pab1 null mutant.\n mYNR064C:Unknown ,, Unknown\n mSET5:,,\n mYNL047C:Unknown ,, Unknown\n mCEG1:mRNA guanylyltransferase (mRNA capping enzyme), alpha subuni\nt,mRNA capping enzyme alpha subunit , mRNA guanylyltransfera\nse,Null mutant is inviable\n mQRI1:UDP-N-acetylglucosamine pyrophosphorylase,UDP-N-acetylglucos\namine pyrophosphorylase,\n mGYP8:Unknown ,, Unknown\n mYOR138C:Unknown ,, Unknown\n mQRI5:Product of gene unknown,,\n mNOT5:member of the NOT complex, a global negative regulator of tr\nanscription,NOT complex member, a global negative regulator \nof transcription,Null mutant is viable, mutations in not4(mo\nt2) are synthetically lethal with mutations in not5, overexp\nression of NOT3 or NOT4(MOT2) suppresses not5 mutations\n mTRS120:targeting complex (TRAPP) component involved in ER to Golgi \nmembrane traffic,,Null mutant is inviable\n mYDR239C:Unknown ,, Unknown\n mSTE18:gamma subunit of G protein coupled to mating factor receptor\ns,G protein gamma subunit , coupled to mating factor recepto\nr,The null mutant is viable but sterile. Sst1 sst2 double mu\ntants and scg1 mutants can be suppressed by a null allele of\n ste18.\n mYGL108C:Unknown ,, Unknown\n mYKL088W:Unknown ,, Unknown\n mRLF2:Chromatin Assembly Complex, subunit 1: largest (p90) subunit\n of three-subunit protein complex (yeast CAF-I) involved in \nDNA-replication-linked nucleosome assembly. Homol. to p150 s\nubunit human Chromatin Assembly Factor-I (CAF-I),chromatin a\nssembly factor-I (CAF-I) p90 subunit,Null mutant is viable, \nsensitive to UV radiation. Rap1 localization is disrupted an\nd silencing of genes adjacent to telomeric DNA is decreased \nin rfl2 mutants.\n mTPM1:tropomyosin I,tropomyosin I,Null mutant is viable, grows slo\nwly, exhibits cell size heterogeneity, has delocalized depos\nition of chitin, mates poorly; exhibits loss of actin cables\n mTOA1:Transcription factor IIA, large chain,large chain , transcri\nption factor IIA subunit,Null mutant is inviable. Overexpres\nsion of TFIIA partially suppresses an spt3 delta mutation. t\noa1 mutants have Spt-phenotypes. spt3 delta toa1 double muta\nnts are inviable.\n mPEX14:Peroxisomal peripheral membrane protein (peroxin) involved i\nn import of peroxisomal matrix proteins,,Null mutant is viab\nle but is unable to grow on oleate and lacks peroxisomes\n mYPL070W:Unknown ,, Unknown\n mRNH70:RNase H(70), a 70 kDa ribonuclease H,ribonuclease H,Null mut\nant is viable.\n mYOL070C:Unknown ,, Unknown\n mMSO1:multicopy suppressor of sec1; small hydrophilic protein, enr\niched in microsomal membrane fraction, interacts with Sec1p,\n,Null mutant is viable, exhibits accumulation of secretory v\nesicles in the bud; mso1 null mutants exhibit double mutant \ninviability in combinaiton with sec1, sec2, and sec4 mutants\n COMP.:Functional classification via a compendium of knockouts. Hug\nes et.al., cell 2000.\n mYHR003C:Unknown ,, Unknown\n mSCL1:Proteasome subunit YC7alpha/Y8 (protease yscE subunit 7),pro\nteasome subunit YC7alpha/Y8 (protease yscE subunit 7),Null m\nutant is inviable, SCL1 is a dominant suppressor of the ts l\nethality of crl3\n mSLA2:Protein involved in membrane cytoskeleton assembly, required\n for cell polarization and endocytosis,transmembrane protein\n,Null mutant is viable and temperature sensitive\n mSTE23:involved in a-factor processing,,effects a-factor secretion \nand mating by a cells\n Cond888:MNNG_2\n mYOR059C:Unknown ,, Unknown\n mYPL267W:Unknown ,, Unknown\n mYLR427W:Unknown ,, Unknown\n mIES1:Hypothetical ORF,,Null mutant is viable\n mPRP3:snRNP from U4/U6 and U5 snRNPs,snRNP from U4/U6 and U5 snRNP\ns,RNA synthesis defective\n mHRB1:an ORF of unknown function located in a centromeric region d\nuplicated between chromosomes III and XIV,hypothetical RNA-b\ninding protein,\n mIES2:Hypothetical ORF,,Null mutant is viable\n mYDR131C:Unknown ,, Unknown\n mNAB2:nuclear polyadenylated RNA binding protein,polyadenylated RN\nA binding protein,Null mutant is inviable\n mAAD14:aryl-alcohol dehydrogenase located on chromosome 14,aryl-alc\nohol dehydrogenase (putative),\n mHBS1:Protein related to translation elongation factor EF-1alpha a\nnd to Suf12p/Sup2p/Gst1p/Sup35p,,\n mYFR039C:Unknown ,, Unknown\n mTRX2:thioredoxin,thioredoxin,Null mutant is viable; trx1-trx2 dou\nble mutant shows prolonged S phase, shortened G(sub)1 and me\nthionine auxotrophy\n mYPR148C:Unknown ,, Unknown\n mCBC2:cap binding complex,nuclear cap binding complex subunit,muta\nnts exhibit promiscuous 3'-end formation; sae-1 mutation cau\nses temporary cell cycle arrest in meiotic prophase\n mYLR225C:Unknown ,, Unknown\n mAPS1:Involved in a subset of clathrin functions at the Golgi,clat\nhrin associated protein complex small subunit,Null mutant is\n viable; aps1 mutants demonstrate synthetic effects with chc\n1 alleles\n mGTR1:Involved in the function of the Pho84 phosphate transporter,\nsmall GTPase (putative),Null mutant is viable but grows slow\nly, is cold-sensitive, and has defects in phosphate uptake\n mPGD1:Probable transcription factor, polyglutamine domain protein,\n,Suppresses hyper-deletion phenotype of hpr1 null mutant; re\nduces frequency of deletions in rad52-1 mutant\n mVPS24:involved in secretion,,Null mutant missorts vacuolar hydrola\nses and accumulates a late endosomal compartment; Class E vp\ns mutant\n mCAF4:CCR4 associated factor,CCR4 transcriptional complex componen\nt,Null mutant is viable\n mYNL129W:Unknown ,, Unknown\n mCTK2:cyclin-related subunit of the kinase complex that phosphoryl\nates the RPO21 CTD (carboxy-terminal domain); also called CT\nDK-I beta subunit,RNA polymerase II C-terminal domain kinase\n beta subunit, similar to cyclin,Null mutant is viable, disp\nlays slow growth and cold sensitive phenotypes in combinatio\nn with ctk1 and ctk3 null mutants\n mCTK3:CTD kinase-I gamma subunit,RNA polymerase II C-terminal doma\nin kinase gamma subunit, similar to cyclin-dependent kinase,\nNull mutant is viable, displays slow growth and cold sensiti\nve phenotypes in combination with ctk1 and ctk2 null mutants\n mYFR003C:Unknown ,, Unknown\n mFAD1:Flavin adenine dinucleotide (FAD) synthetase, which performs\n second step in synthesis of FAD from riboflavin,FAD synthet\nase,Null mutant is inviable\n mYLR290C:Unknown ,, Unknown\n mNTH1:hydrolyzes trehalose; may be inolved in growth transition fr\nom glucose to glycerol; shows significant sequence similarit\ny to Nth2p,neutral trehalase,Null mutant is viable\n mDPB4:DNA Polymerase B (II), 4th subunit,DNA polymerase II (epsilo\nn) 4th subunit,Null mutant is viable\n mMAI1:Unknown ,, Unknown\n mSEH1:Nuclear pore protein, homologous to sec13,,\n Cond891:G1MMS\n mPBS2:Involved in osmoregulation, member of the HOG1 mitogen-activ\nated protein kinase (MAPK) cascade,MAP kinase kinase (MEK) ,\n may act as a scaffolding protein for Sho1p, Ste11p, and Hog\n1p , MAP kinase kinase (MEK) , may act as a scaffolding prot\nein for Sho1p, Ste11p, and Hog1p , MAP kinase kinase (MEK) ,\n may act as a scaffolding protein for Sho1p, Ste11p, and Hog\n1p,Null mutant is viable, sensitive to high osmolarity, sens\nitive to the antibiotic polymyxin B; shows marked decreased \ninduction of transcription by osmotic stress that is mediate\nd by stress response elements; a deletion in RGA1 and PBS2 a\nctivates the pheromone-dependent signal transduction pathway\n independently of the G protein\n mRSC8:Rsc8 is the eighth largest subunit of RSC, a fifteen-protein\n chromatin remodeling complex and related to the Swi/snf Com\nplex.,,Null mutant is inviable\n mYJL184W:Unknown ,, Unknown\n mISY1:Interacts with Syf1p, Prp39p and Ypl213wp. May play a role i\nn mRNA splicing.,,Null mutant is viable and shows a partial \nsplicing defect. isy1 prp19 double mutants are inviable. is\ny1 ntc20 double mutants are very sick and accumulate pre-mRN\nA.\n mVPS4:Defective in vacuolar protein sorting; homologous to mouse S\nKD1 and to human hVPS4,AAA ATPase,Null mutant is viable, exh\nibits protein sorting and morphological defects\n mVPS5:vacuolar Protein Sorting Defective; Golgi retention and vacu\nolar protein sorting,simialr to sorting nexin I,Null mutant \nis viable, missort and secrete soluble vacuolar proteins, co\nntain fragmented vacuoles and mislocalize carboxypepsidase a\nnd Vps10p.\n mSNU71:associated with U1 snRNP (no counterpart in mammalian U1 snR\nNP; contains few SR-, RE- and RD-dipeptides,,Null mutant is \ninviable\n mNBP35:NBP35 encodes an essential evolutionary conserved protein wi\nth homology to bacterial partitioning ATPases,35 kDa nucleot\nide binding protein,Null mutant is inviable\n mCSN12:Unknown ,, Unknown\n Cond643:DES459_(mec1-)_+_0.02%_MMS_-_45_min\n mPPH21:serine-threonine protein phosphatase 2A,,Null mutant is viab\nle, pph21 pph22 mutants produce very small spores in some st\nrain backgrounds and are inviable in others, pph21 pph22 pph\n3 mutants are inviable\n mVPS30:Required for sorting and delivery of soluble hydrolases to t\nhe vacuole.,,Vacuolar hydrolases sorting receptor Vps10p is \nmislocalized in vps30 mutants.\n mMON1:Product of gene unknown,,null mutant is sensitive to monensi\nn and brefeldin A\n mTGL1:triglyceride lipase-cholesterol esterase,cholesterol esteras\ne , triglyceride lipase,\n mGTS1:Protein homologous to Drosophila clock protein,glycine-threo\nnine-serine repeat protein,Null mutant is viable; shows redu\nced lag phase\n mSPC34:Spindle Pole Component of molecular weight 34 kDa,spindle po\nle component,Null mutant is inviable\n mSNU114:involved in splicing,U5 snRNP-specific protein related to EF\n-2,Null mutant is inviable; growth inhibitory when over-expr\nessed; required for pre-mRNA splicing in vivo\n mMMT1:Protein involved in mitochondrial iron accumulation,mitochon\ndrial metal transporter (putative),Null mutant is viable, mm\nt1 mmt2 double deletion mutants exhibit a growth defect on l\now iron medium\n mHSP82:82 kDa heat shock protein; homolog of mammalian Hsp90,heat s\nhock protein 90 , mammalian Hsp90 homolog , heat shock prote\nin 90 , mammalian Hsp90 homolog,Null mutant is viable at 25 \ndegrees C; ability to grow at higher temperatures varies wit\nh gene copy number\n mMCD1:Mitotic Chromosome Determinant; similar to S. pombe RAD21; m\nay function in chromosome morphogenesis from S phase through\n mitosis,,Null mutant is inviable; temperature sensitive mut\nants are defective in mitotic sister chromatid cohesion and \nmitotic chromosome condensation; multicopy suppressor of smc\n1-2 mutation\n mTOS3:Hypothetical ORF,,\n mYAP1:jun-like transcription factor,jun-like transcription factor,\npleiotropic drug resistance\n mTOS5:Hypothetical ORF,,\n mYPL009C:Unknown ,, Unknown\n mIKS1:ira1* kinase suppressor,serine/threonine kinase (putative),N\null mutant is heat shock sensitive\n mYNR068C:Unknown ,, Unknown\n mGOS1:SNARE protein with a C-terminal membrane anchor,,\n mYKL063C:Unknown ,, Unknown\n mAAD6:high degree of similarity with the AAD of P. chrysosporium,a\nryl-alcohol dehydrogenase (putative),Responds to oxidative s\ntress induced by diamide and di-ethyl maleic acid ester in Y\nAP1 dependant manner\n mURE2:Nitrogen catabolite repression regulator that acts by inhibi\ntion of GLN3 in good nitrogen source. Altered form of Ure2p\n creates [URE3] prion.,glutathione transferase (putative) , \nprion , transcriptional regulator,Null mutant is viable but \nexhibits defects in nitrogen catabolite repression (NCR), an\nd null mutant diploids are defective in pseudohyphal growth \nand display an increased incidence of random bud patterns.\n mYGR223C:Unknown ,, Unknown\n mERV1:Protein essential for mitochondrial biogenesis and cell viab\nility,,Null mutant is inviable; mutants demonstrate defects \nin mitochondrial biogenesis\n mRXT1:Unknown ,, Unknown\n mCBT1:Subunit of complex involved in processing of the 3' end of c\nytochrome b pre-mRNA,,Null mutant is viable, shows results i\nn a respiratory deficiency\n mSIW14:Synthetic interaction with Whi2,tyrosine phosphatase,Null mu\ntant fails to show cell cycle arrest upon nutrient starvatio\nn, is sensitive to 5mM caffeine and 1M NaCL, and shows deloc\nalized actin upon nutrient starvation; synthetically lethal \nwith whi2, on minimal medium only\n mAFT2:Activator of Iron (Fe) Transcription,,Null: Deletion of AFT2\n exacerates iron deficiency of AFT1 disruption.\n mSPC42:involved in SPB duplication, may facilitate attachment of th\ne SPB to the nuclear membrane,spindle pole body component,Nu\nll mutant is inviable; temperature sensitive mutations show \nSBP duplication\n mVPS45:Protein of the Sec1p family essential for vacuolar protein s\norting,,Null mutant is viable, defective in the segregation \nof vacuolar material into the developing daughter cell, has \nlarge central vacuoles\n mTFA1:Large subunit of transcription factor tfIIE,transcription fa\nctor tfIIE large subunit,Null mutant is inviable\n mNAT2:Transfers acetyl group from acetyl coenzyme A to the N-termi\nni of proteins beginning with methionine,N alpha-acetyltrans\nferase,Null mutant is inviable\n Cond872:Zero1\n mNAT3:Non-essential gene, growth of null mutants is retarded on bo\nth YPD & YPG media. The mating efficiency of MATalpha cells,\n but not MATa cells, is reduced by nearly three orders of ma\ngnitude.,N-terminal acetyltransferase,Lack of N-terminal ace\ntylation of proteins with Met-Glu-, Met-Asp- and certain oth\ner termini.\n mUME1:Negative regulator of meiosis,transcriptional modulator,Null\n mutant is viable, expression of the meiotic gene IME2 in nu\nll haploid\n mMCT1:malonyl-CoA:ACP transferase,malonyl-CoA:ACP transferase,Null\n mutant is viable, respiratory deficient\n mEND3:Required for endocytosis and organization of the cytoskeleto\nn,,Null mutant is viable and defective in endocytosis\n mRNA15:Protein with a role in mRNA stability and/or poly(A) tail le\nngth,cleavage and polyadenylation factor CF I component invo\nlved in pre-mRNA 3'-end processing,Null mutant is inviable\n mSRB5:subunit of RNA polymerase II holoenzyme/mediator complex,RNA\n polymerase II holoenzyme/mediator subunit,Null mutant is vi\nable\n mNFU1:Nifu-like protein,,Null mutant is viable on YPD 30 degrees C\n, and is synthetically lethal with SSQ1\n mRPN1:Subunit of 26S Proteasome (PA700 subunit),26S proteasome PA7\n00 subunit,Null mutant is inviable; hrd2-1 mutation slows de\ngradation of Hmg2p. hrd2-1 strains are sensitive to canavani\nne and show a global accumulation of ubiquitin-conjugated pr\noteins, but are not temperature-sensitive\n mSCP1:homolog of chicken calponin, thus the name S. cerevisiae Cal\nPonin,calponin homolog,Null mutant is viable and shows no ap\nparent phenotype\n mRPN3:proteasome subunit,26S proteasome regulatory module componen\nt , similar to human p58 subunit,Null mutant is inviable. RP\nN3 is a high copy suppressor of the nin1-1 temperature sensi\ntive phenotype\n mCDC11:involved in proper bud growth,10 nm filament component of mo\nther-bud neck,abnormal cell-wall deposition and bud growth, \ninability to complete cytokinesis, failure to form the ring \nof 10nm filaments in the neck region of budding cells\n mRPN7:Regulatory Particle Non-ATPase, homolog of mammalian proteas\nomal subunit S10/p44,proteasome regulatory particle subunit,\n mRPN8:Regulatory Particle Non-ATPase, homolog of mammalian proteas\nomal subunit S12/p40,proteasome regulatory particle subunit,\n mCDC16:a component of anaphase-promoting complex required for the G\n2/M transition in mitosis and degradation of mitotic cyclins\n, required for sporulation,metal-binding nucleic acid-bindin\ng protein, interacts with Cdc23p and Cdc27p to catalyze the \nconjugation of ubiquitin to cyclin B (putative),Null mutant \nis inviable. cdc16 mutants are unable to progress through th\ne G(sub)2/M transition, cell division cycle blocked at 36 de\ngrees C\n mPRE2:proteasome subunit,proteasome subunit,Null mutant is inviabl\ne, pre2 mutants exhibit defects in chymotrypsin-like proteol\nysis, stress response and ubiquitin signaled protein degrada\ntion\n mPRE3:Proteasome subunit necessary for hydrolysis of peptidylgluta\nmyl-peptide,20S proteasome subunit,Null mutant is inviable\n mMDR1:Mac1-dependent regulator,GTPase activating protein (GAP) fo\nr Ypt6,Null mutant is viable\n mPRE6:alpha-type of subunit of 20S proteasome,20S proteasome alpha\n-type subunit,Null mutant is inviable\n mNHP10:Non-Histone Protein 10,HMG1-box containing protein,null muta\nnt is viable and has normal growth rate\n mSIN4:involved in positive and negative regualtion of transcriptio\nn, possibly via changes in chromatin structure,RNA polymeras\ne II holoenzyme/mediator subunit,Null mutant is viable, temp\nerature sensitive, displays defects in both positive and neg\native regulation of transcription, suppresses Ty insertion m\nutations (Spt-), exhibits decreased superhelical density of \ncircular DNA molecules, exhibits expression from promoters l\nacking UAS elements; associated with a defect in RME1-depend\nent repression and a methionine or cysteine requirement, exh\nibits flocculant/lacy colony morphology, suppressor of snf/s\nwi mutations\n mWHI2:Protein involved in growth regulation,,Null mutant is viable\n mENT2:epsin N-terminal homology-containing protein,,Null mutant is\n viable; synthetically lethal with ent1 (YDL161w). ent2/1 do\nuble mutants have endocytosis and actin cytoskeleton defects\n.\n mENT3:epsin N-terminal homology-containing protein,,unknown\n mPRE9:proteasome component Y13,proteasome component Y13,\n mYGL185C:Unknown ,, Unknown\n mOSH6:Oxysterol Binding Protein,,\n mENT5:Unknown ,, Unknown\n mYPL199C:Unknown ,, Unknown\n mSPT16:global regulator of transcription,,suppression of Ty inserti\non mutations\n mYLR199C:Unknown ,, Unknown\n Cond886:g-ray\n mYKL195W:Unknown ,, Unknown\n mCDC21:cell division cycle blocked at 36 degree C,thymidylate synth\nase,defective in continued replication during S phase of the\n cell cycle; temperature-sensitive thymidylate auxotroph\n mDAD2:Unknown ,, Unknown\n mFOL3:FOLinic acid requiring,dihydrofolate synthetase,Null mutant \nis viable; requires folinic acid for growth\n mVPS60:vacuolar protein sorting (putative),,Null mutant is viable b\nut a class E vps mutant (missorts vacuolar hydrolases and ac\ncumulates late endosomal compartment).\n mSKI6:superkiller; ExtraCellular Mutant; Ribosomal RNA Processing,\nRNAse PH homolog,Null mutant is inviable; mutants show super\nkiller phenotype, improved translation of non-poly(A) mRNA, \nabnormal 60S ribosomal subunits and defective 3' processing \nof 5.8S rRNA; a Tn3 insertion into this gene causes hypersen\nsitivity to the cell surface polymer perturbing agent calcof\nluor white\n mVPS63:Unknown ,, Unknown\n mYFL061W:Unknown ,, Unknown\n mARC15:Arp complex subunit,,Null mutant is viable, but exhibits sev\nere growth defects\n mSPT20:Transcription factor,histone acetyltransferase SAGA complex \nmember , transcription factor,Null mutant is viable, exhibit\ns growth defects on glucose and galactose, fails to grow on \nmedia lacking inositol\n mYPT52:rab5-like GTPase involved in vacuolar protein sorting and en\ndocytosis,,Null mutant is viable; ypt51 ypt52 double deletio\nn exacerbates the temperature sensitivity and vacuolar prote\nin sorting defects of ypt51 deletion\n mMTR2:mRNA transport regulator,mRNA transport regulator,Null mutan\nt is inviable; mtr2 mutants exhibit nuclear mRNA accumulatio\nn and nucleolar fragmentation\n mHLJ1:Homologous to E coli dnaJ protein,,\n mYDR267C:Unknown ,, Unknown\n mUGT51:Udp-glycosyltransferase,UDP-glucose:sterol glucosyltransfera\nse,Null mutant is viable and unable to synthesize sterol glu\ncoside\n mSMD1:Homolog of human core snRNP protein D1, involved in snRNA ma\nturation,,Null mutant is inviable\n mCCT2:cytoplasmic chaperonin of the Cct ring complex related to Tc\np1p; subunit beta,,Null mutant is inviable; some mutant alle\nles exhibit defects in microtubule and actin assembly.\n mCCT3:Homolog of mammalian CCT family of chaperonin proteins; requ\nired for assembly of microtubules and actin in vivo,gamma ch\naperonin subunit,Defects in microtubule and actin assembly i\nn vivo, abberant chromosome segregation, supersensitivity to\n benomyl\n mBET3:Hydrophilic protein that acts in conjunction with SNARE prot\neins in targeting and fusion of ER to Golgi transport vesicl\nes,transport protein particle (TRAPP) component,Null mutant \nis inviable\n mBET4:catalyzes prenylation of Ypt1p (as a subunit of PGGTase-II),\ngeranylgeranyltransferase type II alpha subunit (PGGTase-II,\n alpha subunit),\n Cond884:10\n mHSE1:Unknown ,, Unknown\n mCAK1:binds and phosphorylates Cdc28p,cyclin-dependent kinase-acti\nvating kinase,Null mutant is inviable; temperature-sensitive\n mutant confers a G2 delay accompanied by low Cdc28p protein\n kinase activity\n Jelinski.Jelinski:Regulatory networks revealed by transcriptional profiling of\n damaged Saccharomyces cerevisiae cells: Rpn4 links base exc\nision repair with proteasomes. Mol Cell Biol. 2000 Nov;20(2\n1):8157-67.\n mSGV1:CDC28/cdc2 related protein kinase,CDC28/cdc2 related protein\n kinase,Null mutant is inviable.\n mYJR083C:Unknown ,, Unknown\n mHNT2:Fhit homolog, member of the histidine triad superfamily of n\nucleotide binding-proteins,,\n mGFD1:Great for FULL DEAD box protein activity,,Null mutant is via\nble; high copy suppressor of rat8-2\n mMTD1:NAD-dependent 5,10-methylenetetrahydrafolate dehydrogenase,N\nAD-dependent 5,10-methylenetetrahydrafolate dehydrogenase,Nu\nll mutant is viable, associated with loss of NAD-dependent 5\n,10-methylene-THF dehydrogenase activity and a purine requir\nement in some genetic backgrounds\n mYKL023W:Unknown ,, Unknown\n Cond883:5\n mRPB3:45 kDa subunit of RNA polymerase II,RNA polymerase II 45 kDa\n subunit,Null mutant is inviable; rpb3(ts) mutants at restri\nctive temperature exhibit no assembly of RNA polymerase II\n mRPB4:fourth-largest subunit of RNA polymerase II,RNA polymerase I\nI fourth largest subunit,Null mutant is viable, rbp4 mutants\n are heat and cold sensitive, exhibit slow growth at interme\ndiate temperatures\n mYOR131C:Unknown ,, Unknown\n mCDC46:Member of complex that acts at ARS's to initiate replication\n,,Null mutant is inviable; at nonpermissive temperature cdc4\n6(ts) mutants arrest with a large bud and a single nucleus a\nnd exhibit a high rate of recombination\n mYNR029C:Unknown ,, Unknown\n mCDC48:Microsomal protein of CDC48/PAS1/SEC18 family of ATPases; fu\nll length homology to mammalian protein VCP; involved in sec\nretion, peroxisome formation and gene expression,,Null mutan\nt is inviable\n mNUP49:localizes to discrete spots in the nuclear envelope,nuclear \npore complex subunit,Null mutant is inviable; some nsp1 nsp4\n9 alleles exhibit synthetic lethality\n mTAF10:TFIID subunit (TBP-associated factor) with predicted molecul\nar weight of 23 kD.,TFIID subunit,Null mutant is inviable\n mYFR016C:Unknown ,, Unknown\n mYMR115W:Unknown ,, Unknown\n mYMR289W:Unknown ,, Unknown\n mPPN1:Phosphate metabolism; transcription is regulated by PHO syst\nem,vacuolar polyphosphatase,Null mutant is viable and shows \naccumulation of long chain polyphosphate\n mIWS1:Interacts with Spt6,,Null: Null Mutant is Lethal.\n mCSE2:Protein required for accurate mitotic chromosome segregation\n,RNA polymerase II mediator subcomplex component,Null mutant\n is viable, accumulates large-budded cells, results in signi\nficant increase in chromosome missegregation, slower growth,\n and defective meiosis\n mRTF1:Directly or indirectly regulates the DNA-binding properties \nof Spt15p, the TATA box-binding protein, and the relative ac\ntivities of different TATA elements,nuclear protein,Null mut\nant is viable and can suppress TATA box-binding protein muta\nnts (SPT15) in an allele-specific fashion\n mCSE4:Required for proper kinetochore function; may be involved in\n assembly of a CEN-specific chromatin structure,similar to h\nistone H3 and to human centromere protein CENP-A,Null mutant\n is inviable; cse4-1 mutant causes increased non-disjunction\n of chromosome with mutated CEN and t.s. arrest at G2/M boun\ndary with 2N DNA content\n Cond897:STATMMS\n mHYS2:Putative role in DNA replication,DNA polymerase delta 55 kDa\n subunit,Null mutant is inviable\n Cond894:G2\n mRPN11:Suppressor of mutant (ts on glycerol) tRNA gene deficient in\n the processing of its 3'-end; homologous to S. pombe PAD1 g\nene - global positive regulator of nuclear transcription and\n is involved in maintenance of chromatin structure,,Null mut\nant is inviable\n mABF1:transcriptional activator and ARS1 binding protein,ARS1 bind\ning protein , transcriptional activator , ARS1 binding prote\nin , transcriptional activator , ARS1 binding protein , tran\nscriptional activator,Null mutant is inviable\n mRPN12:Part of 26S proteasome complex that may activate Cdc28p,32-3\n4 kDa protein,Null mutant is inviable; nin1-1 mutant is temp\nerature-sensitive mutant that shows i) higher rates of recom\nbination and chromosome and plasmid loss; ii) greater sensit\nivity to UV irradiation; iii) at restrictive temperature, ar\nrest in G2, failure to activate histone H1 kinase, and accum\nulation of polyubiquinated proteins\n mIKI1:RNA polymerase II Elongator associated protein,,Null mutant \nis viable but is insensitive to pGLK killer toxin; zymotoxin\n resistant; slow growth; thermo-sensitive above 38 0C; caffe\nine, Calcofluor White and 6-azauracil sensitive; G1 cell cyc\nle delay\n mYDR196C:Unknown ,, Unknown\n mYAF9:Yeast homolog of the human leukemogenic protein AF9; member \nof a large protein complex,,Null mutant is viable\n mYNL157W:Unknown ,, Unknown\n mYOR262W:Unknown ,, Unknown\n mUFO1:F-box protein,F-box protein,Null mutant is viable and UV sen\nsitive\n mCWC15:Unknown ,, Unknown\n mTRP3:anthranilate synthase Component II and indole-3-phosphate (m\nultifunctional enzyme),anthranilate synthase component II , \nindole-3-phosphate,Null mutant is viable, tryptophan auxotro\nph\n mMDM12:Required for normal mitochondrial morphology and distributio\nn,mitochondrial outer membrane protein. An Mdm12p homolog ex\nists in S. Pombe which confers a dominant negative phenotype\n when expressed in S. cerevisiae,Null mutant is viable, temp\nerature sensitive, and possesses abnormally large, round mit\nochondria that are defective for inheritance by daughter bud\ns\n mGLC8:Homolog of mammalian protein phosphatase inhibitor 2,protein\n phosphatase 1 (Glc7p) regulator,Null mutant is viable; dele\ntion of glc8 suppresses phenotypes of ipl1 and glc7 mutants\n mMED4:Member of RNA Polymerase II transcriptional regulation media\ntor,RNA polymerase II holoenzyme/mediator subunit,Null mutan\nt is inviable\n mMED7:Member of RNA Polymerase II transcriptional regulation media\ntor,RNA polymerase II holoenzyme/mediator subunit,Null mutan\nt is inviable\n Cond644:DES459_(mec1-)_+_0.02%_MMS_-_60_min\n mRUD3:Relieves uso1-1 transport defect; golgin-160 related protein\n,,Null mutant shows severe growth defect or inviability in c\nombination with many ER-to-Golgi transport mutants, such as \nuso1-1, sec34, sec35-1, sec22-3, and bos1-1. Overproduction \nsuppresses mutations in many of the same genes.\n mSRP54:Signal recognition particle subunit (homolog of mammalian SR\nP54),,Amaya et al. (J. Biochem. (Tokyo) 107:457-463) report \nthat a SRP54 deletion is inviable, whereas Hann and Walter (\nCell 67:131-144) report that SRP54 deletion is viable.\n mRTT106:Regulator of Ty1 Transposition - same phenotype as RTT101 - \nRTT105, disruption causes increase in Ty1 transposition. Iso\nlated from the same screen as the other named RTT genes.,,Nu\nll mutant is viable, but Ty1 retrotransposition is increased\n.\n SGD.GO:Functional classification via a compendium of knockouts. Hug\nes et.al., cell 2000.\n mPRP40:Splicing component that associates with the yeast U1 small n\nuclear ribonucleoprotein particle,U1 snRNP protein,Null muta\nnt is inviable; temperature-sensitive mutants show a splicin\ng defect\n mTAF1:TAFII complex component required for activated transcription\n,,Null mutant is inviable, taf145 (ts) mutants arrest as sma\nll unbudded cells with a G0 like morphology at the nonpermis\nsive temperature\n mRFC1:RFC is a DNA binding protein and ATPase that acts as a proce\nssivity factor for DNA polymerases delta and epsilon and loa\nds proliferating cell nuclear antigen (PCNA) on DNA,replicat\nion factor C subunit 1 , similar to human RFC 140 kDa subuni\nt,Null mutant is inviable, rfc1 conditional mutants arrest b\nefore mitosis\n mPUP2:Proteasome subunit,proteasome subunit,Null mutant is inviabl\ne\n mYHB1:may play a role in the oxidative stress response,flavohemogl\nobin,Null mutant is viable. A rho+ strain carrying a yhb1(-)\n deletion has normal levels of both cyanide-sensitive and cy\nanide-insensitive respiration. Cells that carry a yhb1(-) de\nletion are sensitive to conditions that promote oxidative st\nress.\n mTAF3:TAF(II) complex (TBP-associated protein complex) component,T\nAF(II) complex component,Null mutant is inviable\n mTAF4:Protein required for protein synthesis,TFIID subunit,Null mu\ntant is inviable\n mYNL165W:Unknown ,, Unknown\n mPAN5:ketopantoate reductase,,\n mTAF6:TATA-binding protein-associated-factor,TATA-binding protein-\nassociated-factor,Null mutant is inviable\n mNUP60:nuclear pore protein,nuclear pore complex subunit,\n mPIF1:involved in repair and recombination of mitochondrial DNA; a\nlso plays a role in (nuclear) chromosomal telomere formation\n and elongation,5'-3' DNA helicase,Mitochondrial DNA is heat\n-labile; abnormal telomere formation\n mTAF8:TBP Associated Factor 65 KDa,TFIID subunit,Null mutant is in\nviable\n mCBK1:cell wall biosynthesis kinase,protein kinase,Null mutation i\ns viable; shows alpha factor resistance; in liquid culture l\narge aggregates of cells are formed\n mYLR392C:Unknown ,, Unknown\n mTFG1:Transcription factor TFIIF large subunit,transcription facto\nr TFIIF large subunit,Null mutant is inviable\n mMET7:METhionine requiring,folylpolyglutamate synthetase,Null muta\nnt is viable, requires methionine for growth, and is respira\ntion-deficient\n mTFG2:transcription initiation factor TFIIF middle subunit,transcr\niption initiation factor TFIIF middle subunit,Null mutant is\n inviable\n mMVP1:Protein required for sorting proteins to the vacuole,,MVP1 w\nas identified as a multicopy suppressor of dominant-negative\n vps1 mutations, as well as an extragenic suppressor of a te\nmperature-sensitive pma1 mutation (sop gene)\n mPNG1:de-N-glycosylation enzyme,peptide:N-glycanase,Null mutant is\n viable and shows no growth or viability defect under experi\nmental conditions\n mSRP68:part of the signal recognition particle (SRP) ribonucleoprot\nein (RNP) complex that functions in protein targeting to the\n endoplasmic reticulum (ER) membrane,signal recognition part\nicle component,Null mutant is viable, associated with slow c\nell growth and inefficient protein translocation across the \nER membrane\n mTRS31:targeting complex (TRAPP) component involved in ER to Golgi \nmembrane traffic,,Null mutant is inviable\n mYNL050C:Unknown ,, Unknown\n mYOL036W:Unknown ,, Unknown\n mYNL253W:Unknown ,, Unknown\n mLCB5:involved in sphingolipid biosynthesis,sphingoid long chain b\nase (LCB) kinase,Null mutant is viable, exhibits approximate\nly 97% of wild-type LCB kinase activity; lcb4 lcb5 double de\nletion mutants exhibit no LCB kinase activity\n mRPT2:Probable 26S protease subunit and member of CDC48/PAS1/SEC18\n family of ATPases,,Null mutant is inviable\n mYHC1:U1 snRNP protein required for pre-mRNA splicing,,\n mRPT4:Proteasome Cap Subunit,26S proteasome cap subunit component \n, ATPase , 26S proteasome cap subunit component , ATPase , 2\n6S proteasome cap subunit component , ATPase,Null mutant is \ninviable; ts mutant strain arrests as large-budded cells aft\ner 1, 2, 3 divisions with a G2 content of DNA and a monopola\nr spindle; unduplicated spindle pole body is enlarged as in \nother monopolar mutants; they also fail to arrest at G1 when\n starved for a single amino acid (but do arrest at G1 when d\neprived of all nitrogen), are resistant to cyclohexamide, an\nd are hypersensitive to amino acid analogs, hygromycin B and\n 3-aminotriazole\n mRPT6:member of the 26 S proteasome,ATPase,Null mutant is inviable\n mPSH1:Unknown ,, Unknown\n mYIL112W:Unknown ,, Unknown\n mVTA1:Unknown ,, Unknown\n mCWH41:Glucosidase I, involved in assembly of cell wall beta 1,6 gl\nucan; an ER type II integral membrane N-glycoprotein,glucosi\ndase I,Null mutant is viable, associated with K1 killer toxi\nn-resistant phenotype and a 50% reduction in the cell wall b\neta 1,6-glucan level\n mEMP47:47 kDa type I transmembrane protein localized to the Golgi,4\n7 kDa type I transmembrane protein localized to the Golgi,Nu\nll mutant is viable\n mMKS1:Pleiotropic regulatory factor involved in Ras-CAMP and lysin\ne biosynthetic pathways and nitrogen regulation,negative tra\nnsctiptional regulator,Null mutant is viable, fails to grow \non galactose media containing ethidium bromide at 25 degrees\n and on YPglycerol media at 37 degrees\n Cond898:RPN4\n mPAP1:poly(A) polymerase,poly(A) polymerase,lethal\n Cond889:4NQO_2\n mYHR122W:Unknown ,, Unknown\n mSKO1:Suppressor of PKA overexpression; bZIP protein that binds to\n CRE motifs, interacts with Mig1p,CREB like trancsriptional \nrepressor,Null mutant is viable, associated with partial der\nepression of the SUC2 gene; associated with increased transc\nription through ATF/CREB sites. SKO1 is a multicopy suppress\nor of the lethality caused by overexpressing cAMP-dependent \nprotein kinase and of the toxicity caused by overexpressing \nRap1p\n mNUP84:component of nuclear pores; Part of complex with Nup120p, Nu\np85p, Sec13p, and a Sec13p homolog,similar to mammalian Nup1\n07p,Null mutant is viable but has defects in nuclear membran\ne and nuclear pore complex organization and in poly(A)+ RNA \ntransport\n mNUP85:Protein in nuclear pore complex; may function in nuclear env\nelope integrity; may also be involved in tRNA biogenesis,,Nu\nll mutant is viable but is temperature-sensitive; at nonperm\nissive temperature, null mutant accumulates poly(A)+ RNA and\n has fragmented nucleolus; at permissive temperature, nuclea\nr envelope of null mutant detaches from nucleus\n mMPE1:Unknown ,, Unknown\n mSTS1:restores protein transport when overexpressed and rRNA stabi\nlity to a sec23 mutation,,Null mutant is inviable\n mAPN1:major apurinic/apyrimidinic endonuclease/3'-repair diesteras\ne,major apurinic/apyrimidinic endonuclease/3'-repair diester\nase,hypersensitive to both oxidative and alkylating agents t\nhat damage DNA; higher rate of spontaneous mutation\n mYLR387C:Unknown ,, Unknown\n mRAT1:RNA trafficking protein; transcription activator,5'-3' exori\nbonuclease,Null mutant is inviable.\n mYME1:Mitochondrial protein of the CDC48/PAS1/SEC18 family of ATPa\nses,,Null mutant is viable, exhibits an elevation in the rat\ne at which copies of TRP1 and ARS1, integrated into the mito\nchondrial genome, escape to the nucleus; a heat-sensitive re\nspiratory-growth defect; a cold-sensitive growth defect on r\nich glucose medium; and synthetic lethality in rho- (cytopla\nsmic petite) cells; yme1 (osd1) mutants fail to degrade newl\ny synthesized subunits of cytochrome c\n Cond893:SMMS\n mNIT1:nitrilase,nitrilase,\n mMAS1:mitochondrial processing protease subunit,mitochondrial proc\nessing protease subunit,Null mutant is inviable; Elevated mi\ntotic recombination and chromosomal missegregation when over\nproduced\n mSPP1:YPL138C,,\n mNIT3:Nit protein, nitrilase superfamily member,,\n mHRR25:Similar to YCK1 and YCK2, two other casein kinase I isoforms\n; found primarily in nucleus; may be involved in DNA-damage \nrepair,casein kinase I isoform,Null mutant is viable but sho\nws slow growth; hrr25-1 mutation results in sensitivity to c\nontinuous expression of HO endonuclease, to methylmethanesul\nfonate, and to x-irradiation; homozygous hrr25-1 mutants are\n unable to sporulate\n mMAD1:coiled-coil protein involved in spindle-assembly checkpoint,\n,Null mutant is viable, benomyl sensitive\n mMAD2:spindle checkpoint complex subunit,spindle checkpoint comple\nx subunit,Null mutant is viable, mitotic arrest deficient, s\nensitive to the anti-microtubule drug benomyl\n mYNL335W:Unknown ,, Unknown\n mCET1:Interacts with Ceg1p, the mRNA capping enzyme alpha subunit;\n removes gamma-phosphate from triphosphate-terminated RNA,RN\nA 5'-triphosphatase , mRNA capping enzyme beta subunit (80 k\nDa),Null mutant is inviable\n mBRR1:Protein involved in snRNP biogenesis,spliceosomal snRNP comp\nonent,in brr1 mutants, newly synthesized snRNAs are destabil\nized and 3'-end processing is slowed\n mTLG2:member of the syntaxin family of t-SNAREs,tSNARE that affect\ns a late Golgi compartment,Null mutant is viable in SEY6210,\n exhibits endocytosis defect and loss of Kex2p\n mYDR229W:Unknown ,, Unknown\n mSEC6:cytoplasmic protein involved in fusion of post-Golgi vesicle\ns with the plasma membrane. The Exocyst complex is required \nfor exocytosis.,exocyst complex 88 kDa component,lethal\n mSMY1:not believed to act as a kinesin, colocalizes with Myo2p,kin\nesin heavy chain homolog,high copy suppressor of myosin\n mIST1:Similar to Nuf1p (spindle pole body component),,\n mPUS1:Involved in tRNA biogenesis,tRNA pseudouridine synthase,pus1\n los1 double mutant exhibits loss of suppressor tRNA activit\ny; pus1, los1 and nsp1 mutations cause synthetic lethality\n mSEC9:Putative t-SNARE of the plasma membrane,t-SNARE (putative),A\nn uncharacterized allele accumulates 100nm secretory vesicle\ns and berkeley bodies and is defective in proteint transport\n to the cell surface. The sec9-4 allele has diploid-specific\n bud site selection defects.\n mGCR2:activates transcription of glycolytic genes; homologous to G\nCR1; may function in complex with Gcr1p,transcription factor\n,Null mutant is viable and has partial growth defect on gluc\nose-containing media\n Cond895:G2MMS\n mUFD1:Ubiquitin fusion degradation protein,,Homozygous ufd1-1 muta\nnt diploids exhibit sporulation defects.\n mSEC12:Required for recruitment of Sar1p and vessicle formation at \nthe endoplasmic reticulum.,guanine nucleotide exchange facto\nr for Sar1p,Null mutant is inviable. Defective in endoplasmi\nc reticulum to Golgi transport.\n mYGR130C:Unknown ,, Unknown\n mSUB1:Suppressor of TFIIB mutations,transcriptional coactivator,Nu\nll mutant is viable, auxotrophic for inositol; high copy sup\npressor of SUA7 (TFIIB) mutations. Overexpression of SUB1 st\nimulates transcription by some types of activators in vivo\n mCUS1:cold sensitive U2 snRNA Suppressor,U2 snRNP protein,suppress\nes cold sensitivity of a U2 G53A cs mutant\n Cond877:MMS\n mECO1:Establishment of COhesion,,Null mutant is inviable; temperat\nure-sensitive allele prematurely separates sister chromatids\n, and sister chromatid separation occurs in the absence of f\nunctional APC or Esp1p.\n mIMH1:Product of gene unknown,,Null mutant is viable; imh1 ypt6 do\nuble disruption causes growth inhibition\n mUBP1:Ubiquitin-specific protease,ubiquitin-specific protease,Null\n mutant is viable. Null yuh1 ubp1 ubp2 ubp3 quadruple mutant\ns are viable and retain the ability to deubiquitinate ubiqui\ntin fusions. UBP1 is a dosage dependent suppressor of rsp5 m\nutations\n mADE2:phosphoribosylamino-imidazole-carboxylase,phosphoribosylamin\no-imidazole-carboxylase,Null mutant is viable and requires a\ndenine. ade2 mutants are blocked at a stage in the adenine b\niosynthetic pathway that causes an intermediate to accumulat\ne in the vacuole; the intermediate gives the cell a red colo\nr.\n mVTC2:Phosphate metabolism; transcription is regulated by PHO syst\nem,polyphosphate synthetase (putative),Null nutant is viable\n; no polyphosphate accumulation in a vtc2(phm1)/vtc3(phm2) d\nouble disruptant\n mUBP2:Ubiquitin-specific protease,ubiquitin-specific protease,Null\n mutant is viable. Null yuh1 ubp1 ubp2 ubp3 quadruple mutant\ns are viable and retain the ability to deubiquitinate ubiqui\ntin fusions.\n mPDX1:Plays a structural role in pyruvate dehydrogenase complex,py\nruvate dehydrogenase complex protein X component,Null mutant\n is viable\n mDOP1:homolog of Emericella nidulans developmental regulatory gene\n, dopey (dopA).,,essential gene\n mUBA4:Unknown ,, Unknown\n mYGL164C:Unknown ,, Unknown\n mTWF1:Twinfilin A is a member of a conserved family of actin monom\ner sequestering proteins. TWF1 is comprised almost entirely \nof two tandem repeats, each having sequence homology with co\nfilin (COF1).,twinfilin A, an actin monomer sequestering pro\ntein,Null mutant is viable, twf1 null cof1-22 mutants exhibi\nt synthetic lethality\n mFSH3:Unknown ,, Unknown\n mPLP2:Phosducin-like protein,,Null mutant is inviable\n mYJL123C:Unknown ,, Unknown\n mNGL2:DNase/RNase (putative); CCR4 C-terminal homolog; displays ho\nmology to drosophila Angelgene; homolog to ngl1 and ngl3 ,DN\nase (putative) , RNase (putative),Null mutant is viable.\n Cond885:20\n mCCL1:essential for cell proliferation,TFIIK subunit, a subcomplex\n of transcription factor TFIIH , cyclin,Null mutant is invia\nble\n mCOG8:Unknown ,, Unknown\n mUFE1:t-SNARE that resides on the endoplasmic reticulum and mediat\nes retrograde traffic from the Golgi complex,t-SNARE (ER),Nu\nll mutant is inviable\n mMGM101:Involved in mitochondrial genome maintenance,,Null mutant is\n viable\n mDID4:Hypothetical ORF,class E vacuolar-protein sorting and endocy\ntosis factor,secretion of vacuolar proteins; canavanine-hype\nrsensitive; temperature-sensitive; suppresses defects associ\nated with loss of Doa4\n mYLR345W:Unknown ,, Unknown\n mIOC3:Iswi One Complex,,\n mNKP1:Unknown ,, Unknown\n mIOC4:Iswi One Complex,,\n mORT1:Mitochondrial integral membrane protein, ornithine transport\ner,,Null mutant is viable, arginine bradytroph\n mYLR440C:Unknown ,, Unknown\n mLEO1:Product of gene unknown,,Null mutant is viable\n mYML053C:Unknown ,, Unknown\n
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Computational Genomics Lab, Tel-Aviv uniresity